RayBiotech Introduces Multiplex ELISA Detecting 200 Human Proteins

Norcross, GA, December 12, 2009 --(PR.com)-- RayBiotech, the Protein Array Pioneer Company, has just introduced the Quantibody® Human Cytokine Array 4000 (or Human Q4000, catalog number QAH-CAA-4000), which can quantitatively detect expression of 200 different proteins in a single experiment.

The kit consists of 5 different quantitative multiplex ELISA-based array slides:
• Quantibody® Human Inflammation Array 3 (QAH-INF-3) detects 40 inflammatory markers
• Quantibody® Human Chemokine Array 1 (QAH-CHE-1) detects 40 chemokines
• Quantibody® Human Growth Factor Array 1 (QAH-GF-1) detects 40 growth factors
• Quantibody® Human Receptor Array 1 (QAH-REC-1) detects 40 soluble cytokine receptors
• Quantibody® Human Cytokine Array 4 (QAH-CYT-4) detects 40 cytokines and related proteins

The Quantibody® Human Cytokine Array 4000 array kit is available for purchase immediately, and the constituent arrays listed above are also available for individual purchase. Quantibody® is a glass-slide antibody array platform that uses multiplex ELISA technology using protein standards run along side unknown samples to give quantitative results with picogram-per-milliliter (pg/ml) sensitivity. The Quantibody® format is compatible with a wide range of sample types, including serum, plasma, cell cultured media, cell and tissue lysates, and numerous body fluids.

RayBiotech’s semi-quantitative membrane array has been featured in literally hundreds of biomedical research reports, including publications in Nature, Nature Medicine, Cell, Lancet, Proceedings of the National Academy of Sciences USA, and other top-tier biomedical and scientific journals.

The glass-slide array format, used for the Quantibody® arrays, requires a gene microarray scanner, such as a GenePix from Axon, to detect the fluorescent signal. Fluorescence detection allows for a greater dynamic range of signals, and a smaller sample volume. With the Quantibody® Arrays, 50 microliters of sample can yield quantitative detection of protein expression of up to 40 different proteins simultaneously in a single aliquot.

“This is the most comprehensive multiplex ELISA assay on the market,” said Brett Burkholder, Director of Marketing and Business Development for RayBiotech. “To be able to test for 200 markers and get quantitative data using no more than 250 microliters of serum or plasma in a single experiment, this is unprecedented. No other multiplex ELISA format even comes close.”

RayBiotech Founder and President, Dr. Ray Huang, characterizes this as a breakthrough in antibody-based biomarker discovery: "Previously, antibody-based screening methods were only semi-quantitative, yielding relative expression, or detected only 10 to 20 proteins at a time. Using this product, researchers can now quantitatively screen for 200 biomarkers simultaneously."

Antibodies and standards to quantitatively detect the following proteins are included in this kit:

Quantibody Human Inflammation Array 3 (40 antibody pairs)
BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNF-α, TNF-β, TNF sRI, TNF sRII

Quantibody® Human Chemokine Array 1 (40 antibody pairs)
6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3α, MIP-3β, MPIF-1, MSP-α, NAP-2, OPN, PARC, PF4, SDF-1alpha, TARC, TECK, TSLP

Quantibody® Human Growth Factor Array 8 (40 antibody pairs)
AR, BDNF, basic FGF, BMP-4, BMP-5, BMP-7, β-NGF, EGF, EGF R, EG-VEGF, FGF-4, FGF-7, GDF-15, GDNF, GH, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, Insulin, MCSF R, NGF R, NT-3, NT-4, OPG, PDGF-AA, PIGF, SCF, SCF R, TGF-α, TGF-β1, TGF-β3, VEGF, VEGF R2, VEGF R3, VEGF-D

Quantibody® Human Receptor Array 1 (40 antibody pairs)
4-1BB, ALCAM, B7-1, BCMA, CD14, CD30, CD40L, CEACAM-1, DR6, Dtk, Endoglin, ErbB3, E-Selectin, Fas, Flt-3 Ligand, GITR, HVEM, ICAM-3, IL-1 R4, IL-1 RI, IL-10 Rβ, IL-17R, IL-2Rγ, IL-21R, LIMPII, Lipocalin-2, L-Selectin, LYVE-1, MICA, MICB, NRG1-β1, PDGFR B, PECAM-1, RAGE, TIM-1, TRAIL R3, Trappin-2, uPAR, VCAM-1, XEDAR

Quantibody® Human Cytokine Array 4 (40 antibody pairs)
Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas Ligand, Fcg RIIB/C, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2 Ra, IL-2 Rb, IL-23, LAP (TGF-b1), NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1b, sgp130, Shh N, Siglec-5, ST2/IL1RL-1, TGF-β2, Tie-2, Thrombopoetin, TRAIL R4, TREM-1, VEGF-C, VEGF R1

For More Information, please contact:

Brett Burkholder, Dir. Marketing and Business Development (brett@raybiotech.com)

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RayBiotech, Inc., introduced the first commercially available cytokine antibody array in 2001. Since then, RayBiotech array products have been featured in hundreds of publications, including some in top-tier journals: Nature, Nature Medicine, Cell, Lancet, PNAS (USA), and many others. Offering more antibody array choices than any of its competitors, RayBiotech continues to lead in the development of protein array technologies. A spin-off from Emory University’s School of Medicine, RayBiotech is privately owned, with headquarters in metropolitan Atlanta (Norcross, GA).