MoBiTec Expands Its Selection of High Quality Gene Expression Systems for Bacillus megaterium

Goettingen, Germany, June 09, 2011 --(PR.com)-- MoBiTec GmbH, leading provider of non-Escherichia coli protein expression systems, expands its series of expression vector systems suitable for protein expression in Bacillus megaterium.

Bacillus megaterium is a well established and preferred host for heterologous gene expression and an excellent alternative to the widely used working horse Escherichia coli. B. megaterium is a widely used host for gene expression and does not produce toxic compounds like lipopolysaccharides which makes the purification of produced proteins less tedious and time consuming. The protein yield obtained with the optimized high performance hp vector systems is up to 10 times enhanced compared to protein expression with the respective basic plasmid.

hp vectors are available for intracellular expression, without or with affinity tag for convenient purification:

· pC-His1623hp, E. coli/B. megaterium shuttle vector, C-terminal 6xHis-tag
· pN-His1623hp, E. coli/B. megaterium shuttle vector, N-terminal 6xHis-tag
· p3STOP1623hp, E. coli/B. megaterium shuttle vector

as well as for secretion with 2 different signal peptides:

· pSPLipA-hp, E. coli/B. megaterium shuttle vector with signal sequence
· pSPYocH-hp, E. coli/B. megaterium shuttle vector with signal sequence

Bacillus megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.

For secretion vectors they offer protoplasts of strain MS941. Both strains are mutants of DSM319, whereby WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

· Bacillus megaterium protoplasts, ready for transformation (strain WH320)
· Bacillus megaterium protoplasts, ready for transformation (strain MS941)

About MoBiTec GmbH
MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include two- & one-hybrid systems for in vivo detection of molecular interactions, Lambda PS genomic libraries, DNA vectors for cloning and expression, kits for expression and purification of recombinant proteins, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, gel analysis tools as well as general laboratory equipment. In parallel to its own product lines, MoBiTec distributes products from several international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

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