MoBiTec GmbH, Germany, Offers New Bacillus Megaterium Strain for Gene Expression and Protein Production - YYBm1

Goettingen, Germany, November 13, 2014 --(PR.com)-- Proteins are a group of organic molecules indispensable for life. Over the last decades, the intestinal Gram-negative bacterium Escherichia coli (commonly abbreviated E. coli) has been the preferred host for artificial gene expression. However, its Gram-negative status, giving cause to problems related to the presence of endotoxins, renders it unable to efficiently secrete heterologous proteins into the medium.

The Gram-positive bacterium Bacillus megaterium represents an increasingly used alternative for high yield intra- and extracellular protein production. During the past two decades, multiple tools including gene expression plasmids and production strains have been developed. Introduction of free replicating and integrative plasmids into B. megaterium is possible via protoplasts transformation or transconjugation. Using His- and Strep-affinity tags, the intra- or extracellular produced proteins can easily be purified in one-step procedures. Different gene expression systems based on the xylose controlled promoter P(xylA) and various phage RNA polymerase driven systems (for instance, T7) enable B. megaterium to produce up to 1.25 g of recombinant protein per liter.

MoBiTec GmbH is the only commercial source of gene expression systems for the biotechnologically relevant protein production host Bacillus megaterium.

As of now MoBiTec offers a new host strain for gene expression - YYBm1. Strain YYBm1 is unable to utilize the expression system's inductor molecule xylose as carbon source, thereby possibly making commercial applications more efficient and less expensive. YYBm1 is supplied as ready-for-transformation protoplasts.

For details on the High Performance Expression System for B. megaterium please check:
http://www.mobitec.com/cms/products/bio/04_vector_sys/b_megaterium_hp_expression.html

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.