Two Research Reports Cite HemogloBind™ for Highly Efficient Sample Preparation
Biotech Support Group reports on two recent research reports which describe the simplicity and efficiency of their proteomic sample preparation technology for hemoglobin depletion.
Monmouth Junction, NJ, November 20, 2015 --(PR.com)-- Biotech Support Group reports on two recent research reports which describe the simplicity and efficiency of their proteomic sample preparation technology for hemoglobin depletion.
The first citation is:
Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. Platoff Jr, and David T. Yeung. "Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig." The Journal of Toxicological Sciences 40, no. 6 (2015): 759-775. http://doi.org/10.2131/jts.40.759
https://www.jstage.jst.go.jp/article/jts/40/6/40_759/_article
Acetylcholine is an essential neurotransmitter, and inhibitors of cholinesterases(ChEs) are potent toxins. A primary component of anti-organophosphorus therapy is an oxime reactivator to rescue inhibited acetylcholinesterases. For this, clinical signs of toxicity can be measured from blood cholinesterase [Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] activity utilizing a modified Ellman's method. Biotech Support Group’s unique solid-phase polymer for hemoglobin depletion, was used for pretreatment. The article states “Briefly, whole blood samples were treated with HemogloBind™ which interferes with the ChE activity assay due to spectral overlap.”
The second citation is:
Bhargava, Maneesh. "Proteomic Studies in Acute Hypoxic Respiratory Failure." PhD diss., UNIVERSITY OF MINNESOTA, 2015.
http://conservancy.umn.edu/bitstream/handle/11299/175250/Bhargava_umn_0130E_16432.pdf?sequence=1&isAllowed=y
Protein expression in the bronchoalveolar lavage fluid (BALF) from subjects with acute respiratory distress syndrome (ARDS) was evaluated. The BALF samples were processed by desalting, concentration and removal of high abundance proteins. The protein fractions were trypsin digested and labeled with the iTRAQ reagent for mass spectrometry (MS). The thesis states “BALF samples containing at least 1.2 mg of proteins were processed for LCMS/MS…concentrated and desalted using Amicon 3-MWCO filters. Hemoglobin depletion was performed with HemogloBind™ (Biotech Support Group LLC, Monmouth Junction, NJ) per the manufacturer's instructions.”
Hemoglobin removal (in addition to high abundance protein depletion) improved protein identification to 845 proteins compared to 496 proteins with high abundance protein depletion alone.
“These two reports highlight essential features of HemogloBind™. First is that the separations do not pose any functional or structural changes to the proteins being analyzed. Second is the excellent selectivity profile which allowed almost a 2X increase compared to not having used HemogloBind™, to an impressive total of 845 total proteins identified,” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com
References:
Snider, Thomas H., et al. "Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig." The Journal of Toxicological Sciences40.6 (2015): 759-775.
https://www.jstage.jst.go.jp/article/jts/40/6/40_759/_article
Bhargava, Maneesh. Proteomic Studies in Acute Hypoxic Respiratory Failure. Diss. UNIVERSITY OF MINNESOTA, 2015.
http://hdl.handle.net/11299/175250
The first citation is:
Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. Platoff Jr, and David T. Yeung. "Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig." The Journal of Toxicological Sciences 40, no. 6 (2015): 759-775. http://doi.org/10.2131/jts.40.759
https://www.jstage.jst.go.jp/article/jts/40/6/40_759/_article
Acetylcholine is an essential neurotransmitter, and inhibitors of cholinesterases(ChEs) are potent toxins. A primary component of anti-organophosphorus therapy is an oxime reactivator to rescue inhibited acetylcholinesterases. For this, clinical signs of toxicity can be measured from blood cholinesterase [Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] activity utilizing a modified Ellman's method. Biotech Support Group’s unique solid-phase polymer for hemoglobin depletion, was used for pretreatment. The article states “Briefly, whole blood samples were treated with HemogloBind™ which interferes with the ChE activity assay due to spectral overlap.”
The second citation is:
Bhargava, Maneesh. "Proteomic Studies in Acute Hypoxic Respiratory Failure." PhD diss., UNIVERSITY OF MINNESOTA, 2015.
http://conservancy.umn.edu/bitstream/handle/11299/175250/Bhargava_umn_0130E_16432.pdf?sequence=1&isAllowed=y
Protein expression in the bronchoalveolar lavage fluid (BALF) from subjects with acute respiratory distress syndrome (ARDS) was evaluated. The BALF samples were processed by desalting, concentration and removal of high abundance proteins. The protein fractions were trypsin digested and labeled with the iTRAQ reagent for mass spectrometry (MS). The thesis states “BALF samples containing at least 1.2 mg of proteins were processed for LCMS/MS…concentrated and desalted using Amicon 3-MWCO filters. Hemoglobin depletion was performed with HemogloBind™ (Biotech Support Group LLC, Monmouth Junction, NJ) per the manufacturer's instructions.”
Hemoglobin removal (in addition to high abundance protein depletion) improved protein identification to 845 proteins compared to 496 proteins with high abundance protein depletion alone.
“These two reports highlight essential features of HemogloBind™. First is that the separations do not pose any functional or structural changes to the proteins being analyzed. Second is the excellent selectivity profile which allowed almost a 2X increase compared to not having used HemogloBind™, to an impressive total of 845 total proteins identified,” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com
References:
Snider, Thomas H., et al. "Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig." The Journal of Toxicological Sciences40.6 (2015): 759-775.
https://www.jstage.jst.go.jp/article/jts/40/6/40_759/_article
Bhargava, Maneesh. Proteomic Studies in Acute Hypoxic Respiratory Failure. Diss. UNIVERSITY OF MINNESOTA, 2015.
http://hdl.handle.net/11299/175250
Contact
Biotech Support Group
Ravish Amin
732-274-2866
www.biotechsupportgroup.com
Ravish Amin
732-274-2866
www.biotechsupportgroup.com
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